Tuesday, December 21, 2010

PLA-PEG electrospun gene delivery

The below article discusses the usage of PLA-PEG for electrospinning a scaffold for gene delivery. Find these polymers at www.polyvivo.com, go to the link below or to the original source for full-text for the article. (note the below article did not not necessarily use an Akina product for this research). Abstract follows:

"The present work utilizes electrospinning to fabricate synthetic polymer/DNA composite scaffolds for therapeutic application in gene delivery for tissue engineering. The scaffolds are non-woven, nano-fibered, membranous structures composed predominantly of poly(lactide-co-glycolide) (PLGA) random copolymer and a poly(D,L-lactide)-poly(ethylene glycol) (PLA-PEG) block copolymer. Release of plasmid DNA from the scaffolds was sustained over a 20-day study period, with maximum release occurring at ~2 h. Cumulative release profiles indicated amounts released were approximately 68-80% of the initially loaded DNA. Variations in the PLGA to PLA-PEG block copolymer ratio vastly affected the overall structural morphology, as well as both the rate and efficiency of DNA release. Results indicated that DNA released directly from these electrospun scaffolds was indeed intact, capable of cellular transfection, and successfully encoded the protein [beta]-galactosidase. When tested under tensile loads, the electrospun polymer/DNA composite scaffolds exhibited tensile moduli of ~35 MPa, with ~45% strain initially. These values approximate those of skin and cartilage. Taken together, this work represents the first successful demonstration of plasmid DNA incorporation into a polymer scaffold using electrospinning"

Y.K. Luu, K. Kim, B.S. Hsiao, B. Chu, M. Hadjiargyrou, Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. Journal of Controlled Release 89(2) (2003) 341-353. (http://www.sciencedirect.com/science/article/B6T3D-48CT09D-M/2/c5cb0024a0c3c9a6cbe2acc1452ebac2)

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