FAQ: Is the dye stable enough to use in-vivo?
"Our experience has been the dye is extremely stable and usable in-vivo. It was used previously by Dr. Li's group at University of Kentucky to track PTX nanoparticles (full-text articles at http://tigger.uic.edu/labs/
FAQ: What's the detection limit/Best ratio for imaging?
"It depends on quantity of material, depth of tissue, and quality of imager. FPR749 has a quantum yield of 0.04 but it operates in a very long-wave near-infrared region so there is limited noise from other sources. In Dr. Li's research he used 0.79% w/w FPR749/PTX and was able to track in rat model to specific organs/tumors in an IVIS system. In our research we utilized the ELVIS system which has less depth of permeation than an IVIS system. We used 100% AV06 to make the microparticles for this one and were able to track but this was only SubQ/IM so only a depth of 5-10mm or so.