Tuesday, August 6, 2013

AV06 and FPI749 usage in-vivo imaging

I often get questions about the use of FPI749 as well as conjugates such as AV06 and here's some helpful info on these.

FAQ: Is the dye stable enough to use in-vivo?
"Our experience has been the dye is extremely stable and usable in-vivo. It was used previously by Dr. Li's group at University of Kentucky to track PTX nanoparticles (full-text articles at http://tigger.uic.edu/labs/bpslab/PDF/MolPharmaceutics_8_1985_2011.pdf and http://www.uic.edu/labs/bpslab/PDF/PharmRes_x_y_2013.pdf for similar FPR648). Additionally we uses the AV06 conjugate here to make 50um PLGA microparticles which we injected SubQ and IM in a mouse model and tracked for up to 22 days until euthanasia (results not published but images shown at bottom of website here https://akinainc.com/polyscitech/products/imaging/elvis.php). "

FAQ: What's the detection limit/Best ratio for imaging?
"It depends on quantity of material, depth of tissue, and quality of imager.  FPR749 has a quantum yield of 0.04 but it operates in a very long-wave near-infrared region so there is limited noise from other sources. In Dr. Li's research he used 0.79% w/w FPR749/PTX and was able to track in rat model to specific organs/tumors in an IVIS system.  In our research we utilized the ELVIS system which has less depth of permeation than an IVIS system.  We used 100% AV06 to make the microparticles for this one and were able to track but this was only SubQ/IM so only a depth of 5-10mm or so.
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