Tuesday, May 6, 2014

PLGA probiotic delivery for treatment of bowel disorders

PolySciTech (www.polyscitech.com) provides a wide array of PLGA polymers. Recently PLGA has been co-encapsulated with alginate in order to deliver probiotic and prebiotics orally in a manner which allows them to survive the acidic environment of the stomach so as to treat bowel diseases. Read more: Cook, Michael T., George Tzortzis, Dimitris Charalampopoulos, and Vitaliy V. Khutoryanskiy. "Microencapsulation of a synbiotic into PLGA/alginate multiparticulate gels." International journal of pharmaceutics 466, no. 1 (2014): 400-408. http://www.sciencedirect.com/science/article/pii/S0378517314001860

“Abstract: Probiotic bacteria have gained popularity as a defence against disorders of the bowel. However, the acid sensitivity of these cells results in a loss of viability during gastric passage and, consequently, a loss of efficacy. Probiotic treatment can be supplemented using ‘prebiotics’, which are carbohydrates fermented specifically by probiotic cells in the body. This combination of probiotic and prebiotic is termed a ‘synbiotic’. Within this article a multiparticulate dosage form has been developed, consisting of poly(d,l-lactic-co-glycolic acid) (PLGA) microcapsules containing prebiotic Bimuno™ incorporated into an alginate–chitosan matrix containing probiotic Bifidobacterium breve. The aim of this multiparticulate was that, in vivo, the probiotic would be protected against gastric acid and the release of the prebiotic would occur in the distal colon. After microscopic investigation, this synbiotic multiparticulate was shown to control the release of the prebiotic during in vitro gastrointestinal transit, with the release of galacto-oligosaccharides (GOS) initially occurred over 6 h, but with a triphasic release pattern giving further release over 288 h. Encapsulation of B. breve in multiparticulates resulted in a survival of 8.0 ± 0.3 log CFU/mL cells in acid, an improvement over alginate–chitosan microencapsulation of 1.4 log CFU/mL. This was attributed to increased hydrophobicity by the incorporation of PLGA particles. Abbreviations: dichloromethane, DCM; fluorescein isothiocyanate, FITC; gastrointestinal, GI; galacto-oligosaccharides, GOS; interleukin-6, IL-6; phosphate-buffered saline, PBS; polydispersity index, PDI; poly(d,l-lactic-co-glycolic acid), PLGA; tumor necrosis factor α, TNF-α; Wilkins–Chalgren, WC; Keywords: Prebiotic; Probiotic; Encapsulation; GOS; Galacto-oligosaccharides; Bifidobacterium”

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