PEG-PLA used to improve storage stability of Laccase biocatalyst

PolySciTech (www.polyscitech.com) provides a wide array of PEG-PLA block copolymers.  Recently these copolymers have been utilized to generate an emulsion of Laccase allow for liquid storage of the delicate enzyme for up to 12 days. Read more Moniruzzaman, Muhammad, Yoichiro Nao, Sekhar Bhattacharjee, and Tsutomu Ono. "Laccase Incorporated into PEG-PLA Polymer as Active and Stable Biocatalyst for Ionic Liquids Media." In Applied Mechanics and Materials, vol. 625, pp. 333-336. 2014. http://www.scientific.net/AMM.625.333

“Abstract: Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) [C2mim][PF6] (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330-480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions.”