PolySciTech (
www.polyscitech.com)
provides a wide array of polyester products such as PLA and PLGAs of varying
sizes and monomer ratios. Recently researchers have utilized PLA from
PolySciTech as a means to test the activity of a variety of esterases at
different conditions by measuring the degradation impact these enzymes had on
PLA. Read more: Tchigvintsev, Anatoli, Hai Tran, Ana Popovic, Filip Kovacic,
Greg Brown, Robert Flick, Mahbod Hajighasemi et al. "The environment
shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases
from marine metagenomes." Applied Microbiology and Biotechnology (2014):
1-14. http://link.springer.com/article/10.1007/s00253-014-6038-3
“Abstract: Most of the Earth’s biosphere is cold
and is populated by cold-adapted microorganisms. To explore the natural enzyme
diversity of these environments and identify new carboxylesterases, we have
screened three marine metagenome gene libraries for esterase activity. The
screens identified 23 unique active clones, from which five highly active
esterases were selected for biochemical characterization. The purified
metagenomic esterases exhibited high activity against α-naphthyl and
p-nitrophenyl esters with different chain lengths. All five esterases retained
high activity at 5 °C indicating that they are cold-adapted enzymes. The
activity of MGS0010 increased more than two times in the presence of up to 3.5
M NaCl or KCl, whereas the other four metagenomic esterases were inhibited to
various degrees by these salts. The purified enzymes showed different
sensitivities to inhibition by solvents and detergents, and the activities of
MGS0010, MGS0105 and MGS0109 were stimulated three to five times by the
addition of glycerol. Screening of purified esterases against 89 monoester
substrates revealed broad substrate profiles with a preference for different
esters. The metagenomic esterases also hydrolyzed several polyester substrates
including polylactic acid suggesting that they can be used for polyester
depolymerization. Thus, esterases from marine metagenomes are cold-adapted
enzymes exhibiting broad biochemical diversity reflecting the environmental
conditions where they evolved.”
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