Tuesday, January 13, 2015

PolySciTech PLGA used for microsphere delivery of mesenchymal stem cells in treatment of heart attack

PolySciTech (www.polyscitech.com) provides a wide array of PLGA products. Recently PLGA purchased from PolySciTech was utilized to develop PLGA-PEG microparticles which were subsequently loaded with stem cells and investigated for use in treatment of tissue damage resultant from animal model induced heart attack. Read more: Lee, Young Sook, Kwang Suk Lim, Jung-Eun Oh, Arum Yun, Wan Seok Joo, Hyun Soo Kim, Chae-Ok Yun, and Sung Wan Kim. "Development of porous PLGA/PEI< sub> 1.8 k
biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs)." Journal of Controlled Release (2015). http://www.sciencedirect.com/science/article/pii/S016836591500005X

“Abstract: Multipotent mesenchymal stem cells (MSCs) promise a therapeutic alternative for many debilitating and incurable diseases. However, one of the major limitations for the therapeutic application of human MSC (hMSC) is the lengthy ex vivo expansion time for preparing a sufficient amount of cells due to the low engraftment rate after transplantation. To solve this conundrum, a porous biodegradable polymeric microsphere was investigated as a potential scaffold for the delivery of MSCs. The modified water/oil/water (W1/O/W2) double emulsion solvent evaporation method was used for the construction of porous microspheres. PEI1.8k was blended with poly(lactic-co-glycolic acid) (PLGA) to enhance electrostatic cellular attachment to the microspheres. The porous PLGA/PEI1.8k (PPP) particles demonstrated an average particle size of 290 μm and an average pore size of 14.3 μm, providing a micro-carrier for the MSC delivery. PPP particles allowed for better attachment of rMSCs than non-porous PLGA/PEI1.8k (NPP) particles and non-porous (NP) and porous PLGA (PP) microspheres. rMSC successfully grew on the PPP particles for 2 weeks in vitro. Next, PPP particles loaded with 3 different amounts of hMSC showed increased in vivo engraftment rates and maintained the stemness characteristics of hMSC compared with hMSCs-alone group in rats 2 weeks after intramyocardial administration. These customized PPP particles for MSC delivery are a biodegradable and injectable scaffold that can be used for clinical applications. Keywords: PLGA; Porous microparticle; Mesenchymal stem cell; Human stem cell; Cell therapy; PEI1.8k”


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