PolyVivo block polymers used for nanoparticle study
PolySciTech
Division of Akina, Inc. (www.polyscitech.com)
provides a wide array of biodegradable block copolymers. This includes PolyVivo
block copolymers which are distributed on our behalf by Sigma Aldrich. Recently
these polymers have been used as part of a study in generating drug
encapsulated nanoparticles. Read more: Gu, Frank, Liangfang Zhang, Benjamin A.
Teply, Nina Mann, Andrew Wang, Aleksandar F. Radovic-Moreno, Robert Langer, and
Omid C. Farokhzad. "Precise engineering of targeted nanoparticles by using
self-assembled biointegrated block copolymers." Proceedings of the
National Academy of Sciences 105, no. 7 (2008): 2586-2591. (http://www.pnas.org/content/105/7/2586.full)
“Abstract:
There has been progressively heightened interest in the development of targeted
nanoparticles (NPs) for differential delivery and controlled release of drugs.
Despite nearly three decades of research, approaches to reproducibly formulate
targeted NPs with the optimal biophysicochemical properties have remained
elusive. A central challenge has been defining the optimal interplay of
parameters that confer molecular targeting, immune evasion, and drug release to
overcome the physiological barriers in vivo. Here, we report a strategy for
narrowly changing the biophysicochemical properties of NPs in a reproducible
manner, thereby enabling systematic screening of optimally formulated
drug-encapsulated targeted NPs. NPs were formulated by the self-assembly of an amphiphilic
triblock copolymer composed of end-to-end linkage of
poly(lactic-co-glycolic-acid) (PLGA), polyethyleneglycol (PEG), and the A10
aptamer (Apt), which binds to the prostate-specific membrane antigen (PSMA) on
the surface of prostate cancer (PCa) cells, enabling, respectively, controlled
drug release, "stealth" properties for immune evasion, and
cell-specific targeting. Fine-tuning of NP size and drug release kinetics was
further accomplished by controlling the copolymer composition. By using distinct
ratios of PLGA-b-PEG-b-Apt triblock copolymer with PLGA-b-PEG diblock copolymer
lacking the A10 Apt, we developed a series of targeted NPs with increasing Apt
densities that inversely affected the amount of PEG exposure on NP surface and
identified the narrow range of Apt density when the NPs were maximally targeted
and maximally stealth, resulting in most efficient PCa cell uptake in vitro and
in vivo. This approach may contribute to further development of targeted NPs as
highly selective and effective therapeutic modalities.”
No comments:
Post a Comment