Thursday, June 1, 2017

Fundamental nanoparticle-biological interaction research performed using PLGA-PEG-PLGA from PolySciTech

Although there have been several papers focusing on polymer nanoparticles for drug-delivery applications, there still remains much to be learned about the biological fate of these delivery systems in a fundamental sense aside from specific formulations. Recently, researchers working jointly at University of Ss Cyril and Methodius (Macedonia), CIC biomaGUNE (Spain), Wroclaw University of Science and Technology (Poland), University College Dublin (Ireland), Royal College of Surgeons in Ireland, and Alkaloid AD (Macedonia) utilized PolySciTech (www.polyscitech.com) PLGA-PEG-PLGA block polymers of different sizes (PolyVivo AK017 and PolyVivo AK032) to generate nanoparticles and then assay them for bio-transport and cellular uptake. This fundamental research holds promise to improve nanoparticle delivery systems in general by improving the understanding of their biological interactions. Read more: Dimchevska, Simona, Nikola Geskovski, Rozafa Koliqi, Nadica Matevska-Geskovska, Vanessa Gomez Vallejo, Boguslaw Szczupak, Eneko San Sebastian et al. "Efficacy assessment of self-assembled PLGA-PEG-PLGA nanoparticles: correlation of nano-bio interface interactions, biodistribution, internalization and gene expression studies." International Journal of Pharmaceutics (2017). http://www.sciencedirect.com/science/article/pii/S0378517317304660

“Abstract: The aim of our study was to develop and compare the biological performance of two types of biodegradable SN-38 loaded nanoparticles (NPs) with various surface properties, composed of low and high Mw triblock PLGA-PEG-PLGA copolymers, applying rational quality and safety by design approach. Therefore, along with the optimization of crucial physico-chemical properties and in order to evaluate the therapeutical potential and biocompatibility of prepared polymeric nanoparticles, analysis of nano-bio interactions, cell internalization, gene expression and biodistribution studies were performed. The optimized formulations, one of low Mw and one composed of high Mw PLGA-PEG-PLGA copolymer, exhibited different characteristics in terms of surface properties, particle size, zeta potential, drug loading, protein adsorption and biodistribution, which may be attributed to the variations in nano-bio interface interactions due to different NP building blocks length and Mw. On the contrary to protein adsorption and biodistribution studies, both types of NPs exhibited similar results during cell internalization and gene expression studies performed in cell culture medium containing serum proteins. This pool of useful data for internalization and efficacy as well as the notable advance in the circulation time of low Mw NPs may be further employed for shaping the potential of the designed nanocarriers. Keywords: polymeric nanoparticles; 7-ethyl-10-hydroxycamptotecin (SN-38); PLGA-PEG-PLGA/PEO-PPO-PEO; nanoprecipitation; nano-bio interface interactions; gene expression”
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