Targeted nanoparticles have come to the forefront
recently for their application towards cancer by allowing the particles to bind
to specific sites on tumors. There are many factors, however, which can
interfere with this process and not all of them are well understood yet. Recently,
researchers at Universidade do Porto (Portugal) and Uppsala University (Sweden)
utilized fluorescently-tagged PLGA (PLGA-FKR648, AV015) and PLGA-PEG-Mal
(AI110) from PolySciTech (www.polyscitech.com)
to create Fab-decorated nanoparticles bearing a fluorescent tracer. These
particles were used, along with a series of surfactants, to determine the
impact these surfactants had on the targeting capabilities of these
nanoparticles. This research holds promise to help in generation of more
effective targeted nanoparticle systems by optimizing the surfactant utilized.
Read more: Kennedy, Patrick J., Ines Perreira, Daniel Ferreira, Marika Nestor,
Carla Oliveira, Pedro L. Granja, and Bruno Sarmento. "Impact of
Surfactants on the Target Recognition of Fab-Conjugated PLGA
Nanoparticles." European Journal of Pharmaceutics and Biopharmaceutics
(2018). https://www.sciencedirect.com/science/article/pii/S0939641118301784
“Abstract: Targeted drug delivery with nanoparticles
(NPs) requires proper surface ligand presentation and availability. Surfactants
are often used as stabilizers in the production of targeted NPs. Here, we evaluated
the impact of surfactants on ligand functionalization and downstream molecular
recognition. Our model system consisted of fluorescent poly(lactic-co-glycolic
acid) (PLGA) NPs that were nanoprecipitated in one of a small panel of
commonly-used surfactants followed by equivalent washes and conjugation of an
engineered Fab antibody fragment. Size, polydispersity index and zeta potential
were determined by dynamic light scattering and laser Doppler anemometry, and
Fab presence on the NPs was assessed by enzyme-linked immunosorbent assay. Most
importantly, Fab-decorated NP binding to the cell surface receptor was
monitored by fluorescence-activated cell sorting. 2% polyvinyl alcohol, 1%
sodium cholate, 0.5% Pluronic F127 (F127) and 2% Tween-80 were initially
tested. Of the four surfactants tested, PLGA NPs in 0.5% F127 and 2% Tween-80
had the highest cell binding. These two surfactants were then retested in two
different concentrations, 0.5% and 2%. The Fab-decorated PLGA NPs in 2% F127
had the highest cell binding. This study highlights the impact of common
surfactants and their concentrations on the downstream targeting of
ligand-decorated NPs. Similar principles should be applied in the development
of future targeted nanosystems where surfactants are employed. Keywords: Targeted
nanoparticles; PLGA nanoparticles; surfactant; Fab antibody fragment”
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