PLGA from PolySciTech used in development of novel two-photon fluorescent-tracer nanoparticles

Imaging specific features within living bodies is both critical for diagnostics as well as treatment. This process however is difficult and limited as it applies to obtaining specific details regarding internal features and organs. Recently, researchers at National Central University (Taiwan) used PLGA from PolySciTech (www.polyscitech.com) as part of their development of a novel two-photon fluorescent imaging tracer. This research holds promise for improved diagnostic and imaging techniques. Read more: Cheng, Yu-Min, Chi-Hsiang Lien, Jing-Han Ke, and Fan-Ching Chien. "An excitation wavelength switching to enhance dual-color wide-field temporal-focusing multiphoton excitation fluorescence imaging." Journal of Physics D: Applied Physics (2020). https://iopscience.iop.org/article/10.1088/1361-6463/ab7acc/meta

“Abstract: Dual-color two-photon excitation (TPE)-fluorescence imaging is used in conventional TFMPEM to observe specimens with different fluorophore labels. However, concerns have been raised about the excitation efficiency and selectivity of the fluorophores under fixed-wavelength excitation. This study presents a wavelength-switching approach using a scanning mirror, beam expander, and diffraction grating in the TFMPEM to switch the excitation wavelengths and match the optimal absorption of the fluorophores to acquire dynamic dual-color TPE-fluorescence images. The presented TFMPEM system was demonstrated to have an axial excitation confinement of 2.3–5.0 μm for excitation wavelengths of 730–1000 nm, and was used to visualize three-dimensional images of the vasculature of a mouse brain. The TPE efficiencies of different fluorophores were evaluated through TFMPEM imaging with excitation wavelength scanning to obtain their TPE spectra. Consequently, time-lapsed dual-color TFMPEM imaging was performed on rhodamine 6G (R6G)–poly(lactic-co-glycolic acid) (PLGA) nanoparticles and enhanced-yellow-fluorescent protein (EYFP)-tagged clathrin using excitation wavelengths at the maximum TPEs of R6G and EYFP, respectively. Our results revealed the PLGA-nanoparticle uptake of live cells via long-lived clathrin-coated plaques in clathrin-mediated endocytosis.”

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