PLGA-PEG-Mal from PolySciTech used in research on antibody-targeting of nanoparticles

 

Use of antibodies for generation of targeted nanoparticles is a popular mechanism used within drug-delivery systems to provide for localization of drug molecules. However, since anitbodies have specific 3-dimensional shapes as a result of the various parameters including folding and orientation of the molecule, the manner in which they are attached plays a role in their performance. Recently, researchers at Sungkyunkwan University and Chung-Ang University (Korea) used PLGA-PEG-Mal (AI053) and PLGA (AP041) from PolySciTech to generate reactive nanoparticles for antibody adhesion testing. This research holds promise to improve development of targeted nanoparticles in the future. Lee, Na Kyeong, Chi-Pin James Wang, Jaesung Lim, Wooram Park, Ho-Keun Kwon, Se-Na Kim, Tae-Hyung Kim, and Chun Gwon Park. "Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities." Nano Convergence 8, no. 1 (2021): 1-11. https://nanoconvergencejournal.springeropen.com/articles/10.1186/s40580-021-00274-7

“Abstract: Antibodies have been widely used to provide targeting ability and to enhance bioactivity owing to their high specificity, availability, and diversity. Recent advances in biotechnology and nanotechnology permit site-specific engineering of antibodies and their conjugation to the surfaces of nanoparticles (NPs) in various orientations through chemical conjugations and physical adhesions. This study proposes the conjugation of poly(lactic-co-glycolic acid) (PLGA) NPs with antibodies by using two distinct methods, followed by a comparison between the cell-targeting efficiencies of both techniques. Full-length antibodies were conjugated to the PLGA-poly(ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) NPs through the conventional carbodiimide coupling reaction, and f(ab′)2 antibody fragments were conjugated to the PLGA-poly(ethylene glycol)-maleimide(PLGA-PEG-Mal) NPs through interactions between the f(ab′)2 fragment thiol groups and the maleimide located on the nanoparticle surface. The results demonstrate that the PLGA nanoparticles conjugated with the f(ab′)2 antibody fragments had a higher targeting efficiency in vitro and in vivo than that of the PLGA nanoparticles conjugated with the full-length antibodies. The results of this study can be built upon to design a delivery technique for drugs through biocompatible nanoparticles.”