PLGA from PolySciTech used in development of BMP-2 loaded particles for bone tissue engineering.

 

There are many situations in which bone can be damaged or lost including severe trauma (e.g. car accidents) or diseases such as cancer which necessitate removal of diseased portions. As bone tissue does not naturally regenerate damage which exceeds a certain volume or distance (i.e. a critical sized defect, bone can heal ‘breaks’ between adjacent pieces, but not large holes or amputations) this damage can leave patients with permanent, debilitating injuries. Recently, Gwangju Institute of Science and Technology and Korea Institute of Machinery and Materials (Korea) used PLGA (cat# AP018, AP036) from PolySciTech (www.polyscitech.com) to generate BMP-2 (a protein which induces bone healing) loaded particles and utilized these in a cell-scaffold to provide a structure to enable bone healing. This research holds promise to improve reconstructive surgery for repair of damage to bone tissue caused by trauma or surgery. Read more: Choe, Goeun, Mingyu Lee, Seulgi Oh, Ji Min Seok, Junghyun Kim, Seunghyun Im, Su A. Park, and Jae Young Lee. "Three-dimensional bioprinting of mesenchymal stem cells using an osteoinductive bioink containing alginate and BMP-2-loaded PLGA nanoparticles for bone tissue engineering." Biomaterials Advances (2022): 212789. https://www.sciencedirect.com/science/article/pii/S2772950822000668

“Highlights: We produced bioinks using alginate and poly(lactic-co-glycolic acid) nanoparticles. Composite bioink showed enhanced printability and yielded stable printed constructs. Bone morphogenetic protein-2-loaded nanoparticles achieved 2-week sustained release. The novel bioink significantly promoted osteogenesis of mesenchymal stem cells. Abstract: Hydrogels mimicking the physicochemical properties of the native extracellular matrix have attracted great attention as bioinks for three-dimensional (3D) bioprinting in tissue engineering applications. Alginate is a widely used bioink with beneficial properties of fast gelation and biocompatibility; however, bioprinting using alginate-based bioinks has several limitations, such as poor printability, structural instability, and limited biological activities. To address these issues, we formulated various bioinks using bone morphogenetic protein-2 (BMP-2)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles and alginate for mesenchymal stem cell (MSC) printing and induction of osteogenic differentiation. Incorporation of PLGA nanoparticles into alginate could enhance the mechanical properties and printability of the bioink. In particular, Alg/NPN30 (30 mg/mL PLGA nanoparticles and 3% w/v alginate) was most suitable for 3D printing with respect to printability and stability. BMP-2-loaded PLGA nanoparticles (NPBMP-2) displayed sustained in vitro release of BMP-2 for up to two weeks. Further in vitro studies indicated that bioinks composed of alginate and NPBMP-2 significantly induced osteogenesis of the MSCs compared with other controls, evidenced by enhanced calcium deposition, alkaline phosphatase activity, and gene expression of osteogenic markers. Our novel bioink consisting of widely used biocompatible components displays good printability, stability, and osteogenic inductivity, and holds strong potential for cell printing and bone tissue engineering applications. Graphical abstract: Our novel bioink, consisting of alginate and bone morphogenetic protein-2 (BMP-2)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles, displays good printability, stability, and persistent osteogenic inductivity and will be beneficial for three-dimensional cell printing and bone tissue engineering applications.”