Fundamental nanoparticle-biological interaction research performed using PLGA-PEG-PLGA from PolySciTech
Although
there have been several papers focusing on polymer nanoparticles for
drug-delivery applications, there still remains much to be learned about the
biological fate of these delivery systems in a fundamental sense aside from
specific formulations. Recently, researchers working jointly at University of
Ss Cyril and Methodius (Macedonia), CIC biomaGUNE (Spain), Wroclaw University
of Science and Technology (Poland), University College Dublin (Ireland), Royal
College of Surgeons in Ireland, and Alkaloid AD (Macedonia) utilized
PolySciTech (www.polyscitech.com) PLGA-PEG-PLGA block polymers of
different sizes (PolyVivo AK017 and PolyVivo AK032) to generate nanoparticles
and then assay them for bio-transport and cellular uptake. This fundamental
research holds promise to improve nanoparticle delivery systems in general by
improving the understanding of their biological interactions. Read more: Dimchevska,
Simona, Nikola Geskovski, Rozafa Koliqi, Nadica Matevska-Geskovska, Vanessa
Gomez Vallejo, Boguslaw Szczupak, Eneko San Sebastian et al. "Efficacy
assessment of self-assembled PLGA-PEG-PLGA nanoparticles: correlation of
nano-bio interface interactions, biodistribution, internalization and gene expression
studies." International Journal of Pharmaceutics (2017). http://www.sciencedirect.com/science/article/pii/S0378517317304660
“Abstract: The aim of
our study was to develop and compare the biological performance of two types of
biodegradable SN-38 loaded nanoparticles (NPs) with various surface properties,
composed of low and high Mw triblock PLGA-PEG-PLGA copolymers, applying
rational quality and safety by design approach. Therefore, along with the
optimization of crucial physico-chemical properties and in order to evaluate
the therapeutical potential and biocompatibility of prepared polymeric
nanoparticles, analysis of nano-bio interactions, cell internalization, gene
expression and biodistribution studies were performed. The optimized
formulations, one of low Mw and one composed of high Mw PLGA-PEG-PLGA
copolymer, exhibited different characteristics in terms of surface properties,
particle size, zeta potential, drug loading, protein adsorption and
biodistribution, which may be attributed to the variations in nano-bio
interface interactions due to different NP building blocks length and Mw. On
the contrary to protein adsorption and biodistribution studies, both types of
NPs exhibited similar results during cell internalization and gene expression studies
performed in cell culture medium containing serum proteins. This pool of useful
data for internalization and efficacy as well as the notable advance in the
circulation time of low Mw NPs may be further employed for shaping the
potential of the designed nanocarriers. Keywords: polymeric nanoparticles;
7-ethyl-10-hydroxycamptotecin (SN-38); PLGA-PEG-PLGA/PEO-PPO-PEO;
nanoprecipitation; nano-bio interface interactions; gene expression”
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