Nanoparticles
are small… really small. To put it in perspective, a typical human cell ranges
in size from 30-100 um in diameter. Nanoparticles range in size from 1 um down
to 0.001 um (0.1 um being common) which means that a typical cell is between
300-1000 times the size of a nanoparticle. To put that in perspective, if a
nanoparticle was the size of a kitten (~30 cm) then a human cell would close to
the size of a football field (~90-100 M). One important question in science is
how to get the kitten onto the football field… er… I mean… how to get the
nanoparticle into the cell. This is important because nanoparticles can be
loaded with medicines that have a variety of therapeutic effects which can be
leveraged only if the nano-kitten can make it onto the cellular football field to
make the game-winning kick. There’s two basic ways for either to happen. 1.
Active targeting: For our metaphor we’ll assume nano-kitty has a game-day ticket
which he politely presents at the front gate for entrance. Similarly,
nanoparticles can be specifically conjugated to a specific signal molecule that
has the right configuration to allow the nanoparticle access through the
cellular membrane by accepted channels. (or) 2. Passive-targeting: This simply
relies on the really small nano-kitten simply squeezing through a fence and slipping
onto the football field ‘unseen’ due to its small size. Similarly,
nanoparticles can sometimes enter cells simply because of their small size. A
recent study using PST polymers focused on examining the processes at play in
passive-targeting. In addition to final-products used directly for research,
PolySciTech (www.polyscitech.com)
provides a wide array of intermediates which can be used as precursors for
making the final materials. For example, amine-endcap activated PLGA-NH2 has
the capability to be chemically conjugated to a wide variety of molecules using
common laboratory techniques such as carbodiimide-type conjugation between the PLGA-amine
and a NHS-activated carboxylic acid on the other molecule. Recently,
researchers at Johns Hopkins University utilized PLGA-NH2 (PolyVivo Cat# AI051)
as part of an investigation into nanoparticle transport into living cells. They
took the PLGA-NH2 and conjugated on a ‘caged’ rhodamine dye that did not
fluoresce until it was prepared to do so by exposure to UV-light. This clever
technique allowed the researchers to encapsulate the dye completely within the
nanoparticles and precisely track and characterize the nanoparticles during
cellular uptake studies. This research holds promise to improve nanotherapeutic
formulations for treating a wide-variety of diseases. Read more: Schuster,
Benjamin S., Daniel B. Allan, Joshua C. Kays, Justin Hanes, and Robert L.
Leheny. "Photoactivatable fluorescent probes reveal heterogeneous
nanoparticle permeation through biological gels at multiple scales."
Journal of Controlled Release (2017). http://www.sciencedirect.com/science/article/pii/S0168365917306302
Blog dedicated to answering technical questions in an open format relating to PolySciTech (A division of Akina, Inc.) products.
Tuesday, June 6, 2017
PolySciTech PLGA-NH2 used in study on nanoparticle biotransport: Nanoparticle transport explained using a kitten and a football game.
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