PLGA-PEG-PLGA Thermogel from PolySciTech used in testing epigenetic approach to treating cardiovascular disease

 

Hyperplasia describes the regrowth of vascular tissue within a cardiovascular stent which acts to block off an artery even after it has been stented. Recently, researchers at The Ohio State University and University of Virginia used PLGA-PEG-PLGA (AK012) from PolySciTech (www.polyscitech.com) to form a Thermogel for delivery of various factors which prevent hyperplasia. This research holds promise to provide for improved therapies against cardiovascular disease. Read more: Huang, Yitao, Go Urabe, Mengxue Zhang, Jing Li, Hatice Gulcin Ozer, Bowen Wang, K. Craig Kent, and Lian-Wang Guo. "Nullifying epigenetic writer DOT1L attenuates neointimal hyperplasia." Atherosclerosis 308 (2020): 22-31. https://www.sciencedirect.com/science/article/pii/S0021915020302987

“Highlights: DOT1L and its catalytic products H3K79me2 and H3K79me3 increased due to injury in rat carotid arteries after angioplasty. Silencing DOT1L in vivo inhibited H3K79 methylation and diminished injury-induced intimal hyperplasia. Treatment with a DOT1L inhibitor reduced H3K79 methylation and intimal hyperplasia. Abstract: Background and aims: Histone methyltransferases are emerging targets for epigenetic therapy. DOT1L (disruptor of telomeric silencing 1-like) is the only known methylation writer at histone 3 lysine 79 (H3K79). It is little explored for intervention of cardiovascular disease. We investigated the role of DOT1L in neointimal hyperplasia (IH), a basic etiology of occlusive vascular diseases. Methods and results: IH was induced via balloon angioplasty in rat carotid arteries. DOT1L and its catalytic products H3K79me2 and H3K79me3 (immunostaining) increased by 4.69 ± 0.34, 2.38 ± 0.052, and 3.07 ± 0.27 fold, respectively, in injured (versus uninjured) carotid arteries at post-injury day 7. Dot1l silencing via shRNA-lentivirus infusion in injured arteries reduced DOT1L, H3K79me2, and IH at day 14 by 54.5%, 37.1%, and 76.5%, respectively. Moreover, perivascular administration of a DOT1L-selective inhibitor (EPZ5676) reduced H3K79me2, H3K79me3, and IH by 56.1%, 58.6%, and 39.9%, respectively. In addition, Dot1l silencing and its inhibition (with EPZ5676) in vivo in injured arteries boosted smooth muscle α-actin immunostaining; pretreatment of smooth muscle cells with EPZ5676 in vitro reduced pro-proliferative marker proteins, including proliferating cell nuclear antigen (PCNA) and cyclin-D1. Conclusions: While DOT1L is upregulated in angioplasty-injured rat carotid arteries, either its genetic silencing or pharmacological inhibition diminishes injury-induced IH. As such, this study presents a strong rationale for continued mechanistic and translational investigation into DOT1L targeting for treatment of (re)stenotic vascular conditions.”