PLGA-PEG-Mal from PolySciTech used in development of nanoparticle-based breast cancer therapy

 

The primary limitations on chemotherapeutics are related to their safety profiles as the damage these agents cause to normally healthy tissue is non-trivial and the side effects can be severe. Recently, researchers at Harbin Medical University and Tsinghua University (China) Used mPEG-PLGA (AK037) and Mal-PEG-PLGA (AI020) from PolySciTech (www.polyscitech.com) to create anti-HER2 targeted nanoparticles for targeting of breast cancer. This research holds promise to improve cancer therapies in the future. Read more: Ni, Ling, and You-Xin Li. "Anti-Human Epidermal Growth Factor Receptor 2 Single-Chain Fv Fragment-Decorated DM1 Nanoparticles for Specific Targeting of Human Epidermal Growth Factor Receptor 2-Positive Breast Tumor Cells." Journal of Biomedical Nanotechnology 17, no. 3 (2021): 447-455. https://www.ingentaconnect.com/contentone/asp/jbn/2021/00000017/00000003/art00010

“Abstract: Purpose: Although monoclonal antibodies are used to decorate nanoparticles to target specific cells, penetration of tumor tissues by monoclonal antibodies is limited by their large size. Therefore, we prepared DM1 nanoparticles decorated with the small anti-HER2 single-chain Fv fragment (scFvHER2) of trastuzumab (TMAB) for targeting to human epidermal growth factor receptor 2 (HER2) overexpressing in breast cancer effectively. Methods: ScFvHER2 fragment was coupled with DM1 nanoparticles (NPs) via covalent thiol-maleimide linkages. Their physicochemical properties, uptake by cells, and toxicity to tumor cells were investigated. Their vivo biodistribution was assessed employing liquid chromatographytandem mass spectrometry, while their antitumor activity was investigated in nude mice burdened with BT-474 tumor. Results: Viability of BT-474 cells incubated with scFvHER2-DM1-Nanoparticles (scFv-DM1-NPs) was significantly lower than that of BT-474 cell treated with TMAB-DM1-Nanoparticles (TMAB-DM1-NPs) (P < 0 05). Uptake by cells of scFvDM1-NPs was significantly higher than TMAB-DM1-NPs (P < 0 01). Accumulation of scFv-DM1-NPs in tumor tissue was notably higher than TMAB-DM1-NPs (P < 0 05). scFv-DM1-NPs exhibited improved antitumor effects compared to TMABDM1-NPs (P < 0 05), showing a tumor inhibition rate of more than 70%. Conclusions: ScFvHER2 fragment could serve as a more effective targeting ligand than TMAB, and scFv-DM1-NPs could be developed as a possible drug delivery system to target HER2-positive breast cancer.”