Tuesday, July 20, 2021

PLCL from PolySciTech used in development of 3D Cell-Laden microstructures for tissue engineering applications


Tissue regeneration and tissue engineering applies to processes whereby diseased or damaged tissue is either encouraged to heal or temporarily replaced with a construct which provides for healing. Such a technology can be applied to instances of traumatic damage where normally grafting would be necessary without requiring collection of graft tissue and the limitations associated with this. Recently, researchers at State University of New York at Buffalo used PLCL (AP034, AP074, AP067, and AP142) from PolySciTech (www.polyscitech.com) to create a 3D cell-laden structure by micromachining/manipulation of a cell-seeded 2D surface. This research holds promise to provide for tissue-engineering constructs to aid in healing of damaged tissue. Read More: Chen, Zhaowei, Nanditha Anandakrishnan, Ying Xu, and Ruogang Zhao. "Compressive Buckling Fabrication of 3D Cell‐Laden Microstructures." Advanced Science (2021): 2101027. https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202101027

“Abstract: Tissue architecture is a prerequisite for its biological functions. Recapitulating the three-dimensional (3D) tissue structure represents one of the biggest challenges in tissue engineering. Two-dimensional (2D) tissue fabrication methods are currently in the main stage for tissue engineering and disease modeling. However, due to their planar nature, the created models only represent very limited out-of-plane tissue structure. Here compressive buckling principle is harnessed to create 3D biomimetic cell-laden microstructures from microfabricated planar patterns. This method allows out-of-plane delivery of cells and extracellular matrix patterns with high spatial precision. As a proof of principle, a variety of polymeric 3D miniature structures including a box, an octopus, a pyramid, and continuous waves are fabricated. A mineralized bone tissue model with spatially distributed cell-laden lacunae structures is fabricated to demonstrate the fabrication power of the method. It is expected that this novel approach will help to significantly expand the utility of the established 2D fabrication techniques for 3D tissue fabrication. Given the widespread of 2D fabrication methods in biomedical research and the high demand for biomimetic 3D structures, this method is expected to bridge the gap between 2D and 3D tissue fabrication and open up new possibilities in tissue engineering and regenerative medicine.”

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