Treatment of diseases within the brain, ranging from glioblastoma to Alzheimer's, remains difficult in part due to the blood-brain-barrier. The details of the interactions between proteins and neural cells remain poorly understood which inhibits development of therapies to deliver medicinal molecules into the brain. Researchers at the University of Technology Sydney, The University of Melbourne, and The University of Adelaide used PLGA-PEG-NH2 (cat# AI169) from PolySciTech Division of Akina, Inc. (www.polyscitech.com) to develop nanoparticles covered with cerebrospinal proteins. They used these to research the interactions of such particles with neural cells. This research holds promise to improve drug delivery to brain tissue for treatment of a variety of disease states. Read more: Morshed, Nabila, Claire Rennie, Matthew Faria, Lyndsey E. Collins-Praino, and Andrew Care. "Protein Coronas Derived from Cerebrospinal Fluid Enhance the Interactions Between Nanoparticles and Brain Cells." bioRxiv (2024): 2024-05. https://www.biorxiv.org/content/10.1101/2024.05.31.596763.abstract
“Neuronanomedicine harnesses nanoparticle technology for the treatment of neurological disorders. An unavoidable consequence of nanoparticle delivery to biological systems is the formation of a protein corona on the nanoparticle surface. Despite the well-established influence of the protein corona on nanoparticle behavior and fate, as well as FDA approval of neuro-targeted nanotherapeutics, the effect of a physiologically relevant protein corona on nanoparticle-brain cell interactions is insufficiently explored. Indeed, less than 1% of protein corona studies have investigated protein coronas formed in cerebrospinal fluid (CSF), the fluid surrounding the brain. Herein, we utilize two clinically relevant polymeric nanoparticles (PLGA and PLGA-PEG) to evaluate the formation of serum and CSF protein coronas. LC-MS analysis revealed distinct protein compositions, with selective enrichment/depletion profiles. Following incubation with brain cells, serum and CSF coronas on PLGA particles showed enhanced associations with all cell types as compared to their corresponding corona on PLGA-PEG particles. CSFderived protein coronas on PLGA nanoparticles, specifically, showed the greatest nanoparticle-cell interactions, with Pearson’s correlation analysis revealing that proteins associated with enhanced nanoparticle-cell interactions were exclusively enriched in this protein corona. This study demonstrates the importance of correct choice of physiologically relevant biological fluids, and its influence on the formation of the protein corona, subsequent nanoparticle-cell interactions. Keywords: protein corona; bio-nano interactions, neuronanomedicine; cerebrospinal fluid; neurons; glia; targeted drug delivery”
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