Fluorescent PLGA-Rhodamine from PolySciTech used in development of siRNA loaded nanoparticles

 

siRNA holds promise to provide for selective shut-off genes and can be used to treat a variety of disease states. Despite this, delivery of this class of molecules remains a challenge. Researchers at University of Naples Federico II and Tel Aviv University used PLGA-Rhodamine (cat# AV011 https://akinainc.com/polyscitech/products/polyvivo/index.php?highlight=AV011#h) from PolySciTech division of Akina, Inc. as part of development of a nanoparticle delivery system for siRNA. This research holds promise to unlock the usage of this class of pharmaceutics. Read more: Longobardi, Giuseppe, Pini Shekhter, Claudia Conte, Ronit Satchi-Fainaro, and Fabiana Quaglia. "Double-coated PLGA nanoparticles with hierarchical surface architecture for CD44-targeted siRNA delivery." Drug Delivery and Translational Research (2026): 1-16. https://link.springer.com/article/10.1007/s13346-026-02115-8

“Efficient delivery of small interfering RNA (siRNA) remains a materials challenge because it requires nanocarriers that stabilize polyanionic cargo, support cellular interactions, and enable cytosolic delivery. Although poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) are used due to biocompatibility, biodegradability, and regulatory acceptance, siRNA delivery with PLGA requires interfacial engineering to meet these constraints. Here, a modular double-coated PLGA NP platform (dcNPs2.0) is developed and optimized for siRNA complexation, surface functionalization, and scalable manufacturing. The system comprises a PLGA core coated with a polyethyleneimine (PEI) interlayer to mediate siRNA binding, followed by a hyaluronic acid (HA) outer layer, which improves colloidal stability and promotes CD44-mediated uptake. Process optimization, including transition from batch nanoprecipitation to microfluidic fabrication, provides high yield, excellent reproducibility, narrow size distributions, and increased siRNA loading. X-ray photoelectron spectroscopy confirms hierarchical multilayer assembly. The optimized dcNPs2.0 formulation exhibited robust physicochemical stability during storage, in serum-containing media, and following lyophilization with appropriate cryoprotection. Functional evaluation of dcNPs2.0 demonstrated efficient HA-mediated cellular uptake and effective silencing following siRNA delivery in both two-dimensional monolayers and three-dimensional spheroids of MDA-MB-231 cells. Overall, this work establishes a scalable, rationally engineered PLGA nanoplatform that integrates extracellular targeting with intracellular delivery requirements for siRNA therapeutic applications. Keywords: PLGA nanoparticles, Surface engineering, siRNA delivery, CD44-mediated targeting, 3D models”

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