Friday, November 5, 2010

Protein delivery via PEG-PLA

The below study highlights the capabilities for PEG-PLA to be used for delivery of protein based BMP molecules. Check out http://www.polyvivo.com to learn more. (note the below study did not necessarily use a PolySciTech product(s)). Please go to original source for fultext or follow the link below. Abstract follows:

Bone morphogenetic proteins (BMPs) are biologically active molecules capable of eliciting new bone formation. In combination with biomaterials, these proteins can be used in a clinical setting as bone-graft substitutes to promote bone repair. To find new synthetic absorbable polymers with plastic nature that can be used as BMP-carrier materials, six types of poly-D,L-lactic acid-polyethylene glycol block copolymer (PLA-PEG) with various molecular weights of PLA and PEG were synthesized. These were PLA6,500-PEG3,000 (P-1), PLA11,500-PEG3,000 (P-2), PLA17,500-PEG3,000 (P-3), PLA6,500-PEG1,000 (P-4), PLA15,000-PEG8,000 (P-5), and PLA8,500-PEG1,000 (P-6). Fifty milligrams of these polymers was mixed with 0 μg (control) or 5, 10, or 20 μg of recombinant human BMP-2 (rhBMP-2). These pellets were implanted into the dorsal muscle pouches of 144 mice (six pellets consisting of the same polymer and dose of rhBMP-2 for a specific group). Three weeks after surgery, the pellets were harvested and examined by radiographic and histological methods. All P-1 pellets with 10 or 20 μg of rhBMP-2 showed bone formation with hematopoietic marrow and bony trabeculae, as did one third of those with 5 μg of rhBMP-2. The incidence of new bone formation with P-2 pellets or that of P-5 pellets was lower than that of P-1 pellets. No bone was formed in any other type of pellet. These results indicated that the PLA6,500-PEG3,000 polymer with plastic properties was found to work well as a BMP carrier.

N. Saito, T. Okada, S. Toba, S. Miyamoto, K. Takaoka, New synthetic absorbable polymers as BMP carriers: Plastic properties of poly-D,L-lactic acid-polyethylene glycol block copolymers. Journal of Biomedical Materials Research 47(1) (1999) 104-110.
http://dx.doi.org/10.1002/(SICI)1097-4636(199910)47:1<104::aid-jbm15>3.0.CO;2-7

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