Friday, November 5, 2010

Protein Adsorbtion for diblock PEG-PLGA/PLA

The below study highlights the capabilities for dilock PEG-PLGA/PLA to be used for controlling protein adsorption/cellular behavior on surfaces. Check out http://www.polyvivo.com to learn more. (note the below study did not necessarily use a PolySciTech product(s)). Please go to original source for fultext or follow the link below.

abstract:
The adhesion of marrow stromal osteoblasts and the adsorption of fetal bovine serum (FBS) proteins to end-capped poly(D,L-lactic acid) 50:50 (PLA50) of molecular weight 17,000 (PLA5017), non-end-capped PLA50 of molecular weight 11,000 (PLA5011h), and a diblock copolymer made of poly(ethylene glycol)-monomethyl ether of molecular weight 5,000 and PLA50 of molecular weight 20,000 (Me.PEG5–PLA20) were investigated. Cell attachment and proliferation on both PLA50 polymers were equally good. The block copolymer did not allow the proliferation of cells. However, the attached cells were highly differentiated and metabolically active in contrast to the cells on PLA50. Moreover, surface analysis studies using electron spectroscopy revealed that FBS proteins adsorbed well from aqueous solutions to the PLA50 surfaces while they adsorbed substantially less to the block copolymer. These results suggest that Me.PEG-PLA block copolymers may be used to regulate protein adsorption and, therefore, cell adhesion by varying the block composition of the copolymer.

http://dx.doi.org/10.1002/(SICI)1097-4636(19990905)46:3<390::aid-jbm12>3.0.CO;2-N
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