Wednesday, February 20, 2013

PLGA density?

Often I get questions about the density of PLGA which is a reasonable request especially for doing volumetric calculations.  The good news is, generally speaking, the density of PLGA changes relatively little with MW and ratio changes. Whenever I do calculations on this I use 1.58 g/ml and this is based on reported literature value for PLGA 50:50, about ~20kDa, acid endcap as shown below.
Blasi, P., D'Souza, S. S., Selmin, F., & DeLuca, P. P. (2005). Plasticizing effect of water on poly (lactide-co-glycolide). Journal of controlled release, 108(1), 1-9.
"The purpose of this research was to evaluate the effect and nature of hydration on the glass transition temperature (Tg) of poly(d,l-lactide-co-glycolide) and investigate the physical state of water within the polymer during hygrothermal aging. The polymer was incubated in water at 23, 30, 37 and 55 °C, while the vapor sorption studies were carried out at 37 °C using saturated salt solutions. The water content and the thermal behavior of PLGA-water system were assessed by Karl Fischer titration and modulated differential scanning calorimetry, respectively, the hygrothermal aging was monitored by gel permeation chromatography. Water depressed reversibly the Tg by about 15 °C regardless of the incubation conditions. The Tg then remained constant at ∼30 °C for five days, except when degradation occurred. A broad ice melting peak was detected around 0 °C. In the sorption studies, a linear correlation (r2 0.9837) between the Tg and the moisture content was observed in the range of 0.3–2.6% w/w, but there was no discernible endothermic event associated with the melting of ice. Data were found to fit reasonably well to the Gordon–Taylor/Kelley–Bueche equation. There were no differences between bulk and vapor water aging. It is proposed that the water responsible for plasticizing the polymer was non-freezable (bound) water and the small fraction of such water which was absorbed at high relative humidity caused polymer degradation in the same manner as bulk water."
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