Thursday, April 10, 2014

mPEG-PLGA for delivery of c-Myc siRNA glioma treatment

PolySciTech (www.polyscitech.com) provides a variety of mPEG-PLGA’s such as PolyVivo AK02 (2000-4000Da) and AK10 (5000-10,000Da).  Recent research has shown that mPEG-PLGA is a good nanoparticle carrier of silencing RNA for c-Myc gene and serves to promote aptosis of glioma as a potential treatment for brain-cancer.  Read more: Ma, Tao, Jin-Ling Jiang, Ying Liu, Zheng-Bao Ye, and Jun Zhang. "Preparation and evaluation of nanoparticles loading plasmid DNAs inserted with siRNA fragments targeting c-Myc gene." Pharmaceutical biology 0 (2014): 1-10. http://informahealthcare.com/doi/abs/10.3109/13880209.2014.880489


“Abstract: Context: c-Myc plays a key role in glioma cancer stem cell maintenance. A drug delivery system, nanoparticles loading plasmid DNAs inserted with siRNA fragments targeting c-Myc gene (NPs-c-Myc-siRNA-pDNAs), for the treatment of glioma, has not previously been reported. Objective: NPs-c-Myc-siRNA-pDNAs were prepared and evaluated in vitro. Materials and methods: Three kinds of c-Myc-siRNA fragments were separately synthesized and linked with empty siRNA expression vectors in the mole ratio of 3:1 by T4 DNA ligase. The linked products were then separately transfected into Escherichia coli. DH5α followed by extraction with Endofree plasmid Mega kit (Qiagen, Hilden, Germany) obtained c-Myc-siRNA-pDNAs. Finally, the recombinant c-Myc-siRNA3-pDNAs, generating the highest transfection efficiency and the greatest apoptotic ability, were chosen for encapsulation into NPs by the double-emulsion solvent-evaporation procedure, followed by stability, transfection efficiency, as well as qualitative and quantitative apoptosis evaluation. Results: NPs-c-Myc-siRNA3-pDNAs were obtained with spherical shape in uniform size below 150 nm, with the zeta potential about −18 mV, the encapsulation efficiency and loading capacity as 76.3 ± 5.4% and 1.91 ± 0.06%, respectively. The stability results showed that c-Myc-siRNA3-pDNAs remained structurally and functionally stable after encapsulated into NPs, and NPs could prevent the loaded c-Myc-siRNA3-pDNAs from DNase degradation. The transfection efficiency of NPs-c-Myc-siRNA3-pDNAs was proven to be positive. Furthermore, NPs-c-Myc-siRNA3-pDNAs produced significant apoptosis with the apoptotic rate at 24.77 ± 5.39% and early apoptosis cells observed. Discussion and conclusion: Methoxy-poly-(ethylene-glycol)-poly-(lactide-co-glycolide) nanoparticles (MPEG–PLGA-NPs) are potential delivery carriers for c-Myc-siRNA3-pDNAs. Keywords: Apoptosis, double-emulsion solvent-evaporation, flow cytometry, glioma, MPEG–PLGA, transfection”
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