PolySciTech (
www.polyscitech.com) provides a wide
array of mPEG-PLGA block copolymers. Recently research has shown these types of
polymers have the capacity to form stable nanoparticles loaded with c-Myc SiRNA
in order to prevent DNAse degradation and aid delivery of this silencer to
treat glioma cells. Read more: Ma, Tao, Jin-Ling Jiang, Ying Liu, Zheng-Bao Ye,
and Jun Zhang. "Preparation and evaluation of nanoparticles loading
plasmid DNAs inserted with siRNA fragments targeting c-Myc gene."
Pharmaceutical biology 0 (2014): 1-10. http://informahealthcare.com/doi/abs/10.3109/13880209.2014.880489
“Abstract: Context: c-Myc plays a key role in
glioma cancer stem cell maintenance. A drug delivery system, nanoparticles
loading plasmid DNAs inserted with siRNA fragments targeting c-Myc gene
(NPs-c-Myc-siRNA-pDNAs), for the treatment of glioma, has not previously been
reported. Objective: NPs-c-Myc-siRNA-pDNAs were prepared and evaluated in
vitro. Materials and methods: Three kinds of c-Myc-siRNA fragments were
separately synthesized and linked with empty siRNA expression vectors in the
mole ratio of 3:1 by T4 DNA ligase. The linked products were then separately
transfected into Escherichia coli. DH5α followed by extraction with Endofree
plasmid Mega kit (Qiagen, Hilden, Germany) obtained c-Myc-siRNA-pDNAs. Finally,
the recombinant c-Myc-siRNA3-pDNAs, generating the highest transfection
efficiency and the greatest apoptotic ability, were chosen for encapsulation
into NPs by the double-emulsion solvent-evaporation procedure, followed by
stability, transfection efficiency, as well as qualitative and quantitative apoptosis
evaluation. Results: NPs-c-Myc-siRNA3-pDNAs were obtained with spherical shape
in uniform size below 150 nm, with the zeta potential about −18 mV, the
encapsulation efficiency and loading capacity as 76.3 ± 5.4% and 1.91 ± 0.06%,
respectively. The stability results showed that c-Myc-siRNA3-pDNAs remained
structurally and functionally stable after encapsulated into NPs, and NPs could
prevent the loaded c-Myc-siRNA3-pDNAs from DNase degradation. The transfection
efficiency of NPs-c-Myc-siRNA3-pDNAs was proven to be positive. Furthermore,
NPs-c-Myc-siRNA3-pDNAs produced significant apoptosis with the apoptotic rate
at 24.77 ± 5.39% and early apoptosis cells observed. Discussion and conclusion:
Methoxy-poly-(ethylene-glycol)-poly-(lactide-co-glycolide) nanoparticles (MPEG–PLGA-NPs)
are potential delivery carriers for c-Myc-siRNA3-pDNAs. Keywords: Apoptosis,
double-emulsion solvent-evaporation, flow cytometry, glioma, MPEG–PLGA,
transfection”
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