PolySciTech (www.polyscitech.com)
provides a wide array of PLGA and PCL polymers. Recently these types of
polymers were investigated as part of a stem-cell loaded scaffold for use in
regenerating lost bone materials in thumb or fingers. As part of the study
human bone was grown on these scaffolds in mice and tested for its strength and
for bone tissue formation. Read more: Weinand, C., E. Weinberg, C. M. Neville,
R. Gupta, F. Shapiro, and J. P. Vacanti. "LOP29: Human Stem Cells and
Hydrogel β-TCP/PCl versus Hydrogel β-TCP/PLGA Scaffolds in Human Thumb
Regeneration." Plastic and Reconstructive Surgery 134, no. 2 (2014): 389. http://journals.lww.com/plasreconsurg/Abstract/2014/08000/LOP29___Human_Stem_Cells_and_Hydrogel___TCP_PCl.98.aspx
“Abstract: Introduction: The absence of the thumb from
either trauma or congenital defect renders a patient in severe disability and
loss of function in daily life. What is the best B-TCP based biomaterial for
tissue engineering human thumb bones? Material and Methods: In our experiment,
we used three-dimensional printed (3DP) scaffolds from a CT scan of a human
distal phalanx to test ability to support bone formation in vivo. Human mesenchymal stem cells (hMSCs) were
expanded, suspended in collagen I and fibrin glue hydrogel and applied onto 3DP
B-TCP/PCL or B-TCP/PLGA scaffolds. Acellular constructs and scaffolds only
served as controls. Constructs were implanted subcutaneously into nude mice for
6 weeks. Samples were then evaluated using high resolution VCT scanning,
histologically by Toluidin blue, van Kossa, and alkaline phosphatase stain and
biomechanically. Results: In vivo high resolution VCT scanning revealed
densities closer to native bone in cellular B-TCP/PLGA specimens than in
B-TCP/PCL specimens. Histologically collagen I hydrogel B-TCP/PLGA specimens
had superior bone tissue, although radiopacities were detected in collagen I
and Fibrin glue B-TCP/PCL samples. Biomechanical compression testing, however
showed higher stiffness in cellular B-TCP/PCL collagen I than in B-TCP/PLGA.
Expression of bone specific proteins was highest in cellular B-TCP/PLGA
collagen I specimens. Statistical analysis confirmed high correlations between
volumetric CT and biomechanical values and expression of bone specific
proteins. Conclusion: This new approach could be potentially used in the
surgical reconstruction for patients with bone loss of the hand.”
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