PolySciTech (
www.polyscitech.com)
provides a wide array of PEG-PLGA copolymers. Recently these types of polymers
have been used to develop an anti-cancer micelle which delivers docetaxel to
the cancer cells and the delivery is improved by co-administration with
chloroquine to reduce cellular lysosome degradation of the micelle. Read
more: Zhang, Xudong, Xiaowei Zeng, Xin
Liang, Ying Yang, Xiaoming Li, Hongbo Chen, Laiqiang Huang, Lin Mei, and
Si-Shen Feng. "The chemotherapeutic potential of PEG- b-PLGA copolymer
micelles that combine chloroquine as autophagy inhibitor and docetaxel as an
anti-cancer drug." Biomaterials (2014). http://www.sciencedirect.com/science/article/pii/S0142961214008370
“Abstract: Micelles may be the nanocarrier that
is used most often in the area of nanomedicine due to its promising performance
and technical simplicity. However, like the original drugs, micellar
formulation may arouse intracellular autophagy that deteriorates their
advantages for efficient drug delivery. There has been no report in the
literature that involves the fate of micelles after successfully internalized
into the cancer cells. In this study, we show by using docetaxel-loaded
PEG-b-PLGA micelles as a micellar model that the micelles do arouse
intracellular autophagy and are thus subject to degradation through the
endo-lysosome pathway. Moreover, we show that co-administration of the micellar
formulation with autophagy inhibitor such as chloroquine (CQ) could significantly
enhance their therapeutic effects. The docetaxel-loaded PEG-b-PLGA micelles are
formulated by the membrane dialysis method, which are of 7.1% drug loading and
72.8% drug encapsulation efficiency in a size range of around 40 nm with narrow
size distribution. Autophagy degradation and inhibition are investigated by
confocal laser scanning microscopy with various biological makers. We show that
the IC50 values of the drug formulated in the PEG-b-PLGA micelles after 24 h
treatment MCF-7 cancer cells with no autophagy inhibitor or in combination with
CQ were 22.30 ± 1.32 and 1.75 ± 0.43 μg/mL respectively, which indicated a
12-fold more efficient treatment with CQ. The in vivo investigation further
confirmed the advantages of such a strategy. The findings may provide advanced
knowledge for development of nanomedicine for clinical application. Keywords:
Biodegradable polymers; Cancer nanotechnology; Chemotherapeutic engineering;
Intracellular autophagy; Nanomedicine; Pharmaceutical nanotechnology”
No comments:
Post a Comment