Friday, September 5, 2014

New PEG-PLA-FKR648 from PolySciTech for NIR tracking of micelles

PolySciTech (www.polyscitech.com) provides a wide variety of fluorescently conjugated polyesters such as PLGA-FITC and others. We are proud to announce the development of our first fluorescently conjugated block copolymer. The block copolymer, PolyVivo AV17, is same size and configuration as our popular AK09 (mPEG-P(DL)La 2000-2200 Da) but has a NIR fluorescent dye (Flamma Fluor FKR-648) conjugated to the P(DL)La alcohol terminus via a dichlorotriazine linkage.  Read more about appropriate NIR imaging conditions for this type of dye here. Full-Text: Hollis, Christin P., Heidi L. Weiss, B. Mark Evers, Richard A. Gemeinhart, and Tonglei Li. "In Vivo Investigation of Hybrid Paclitaxel Nanocrystals with Dual Fluorescent Probes for Cancer Theranostics." Pharmaceutical research 31, no. 6 (2014): 1450-1459. http://www.uic.edu/labs/bpslab/PDF/PharmRes_31_1450_2014.pdf

“Abstract: To develop novel hybrid paclitaxel (PTX) nanocrystals, in which bioactivatable (MMPSense® 750 FAST) and near infrared (Flamma Fluor® FPR-648) fluorophores are physically incorporated, and to evaluate their anticancer efficacy and diagnostic properties in breast cancer xenograft murine model. The pure and hybrid paclitaxel nanocrystals were prepared by an anti-solvent method, and their physical properties were characterized. The tumor volume change and body weight change were evaluated to assess the treatment efficacy and toxicity. Bioimaging of treated mice was obtained non-invasively in vivo.The released MMPSense molecules from the hybrid nanocrystals were activated by matrix metalloproteinases (MMPs) in vivo, similarly to the free MMPSense, demonstrating its ability to monitor cancer progression. Concurrently, the entrapped FPR-648 was imaged at a different wavelength. Furthermore, when administered at 20 mg/kg, the nanocrystal formulations exerted comparable efficacy as Taxol®, but with decreased toxicity. Hybrid nanocrystals that physically integrated two fluorophores were successfully prepared from solution. Hybrid nanocrystals were shown not only exerting antitumor activity, but also demonstrating the potential of multi-modular bioimaging for diagnostics.”


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