PolySciTech
division of Akina, Inc. (www.polyscitech.com)
provides a wide array of biodegradable block copolymers including thermogelling
PLGA-PEG-PLGA block copolymers. Recently, PolySciTech’s thermogelling PolyVivo
AK097, was used by researchers at Auburn University for the co-administration
of a phage-based vaccine as an adjuvant. Impressively, after 4-8 weeks this
co-administration of phage with thermogel yielded a statistically significant
2.5-3 fold increase in the immune response (as measured by ELISA) relative to a
control delivery of the phage itself without the thermogel (Fig 6A). This
indicates that the thermogel has promise as an adjuvant to boost the immune
response towards vaccines and improve therapeutic efficacy of these vaccines. Read
more: Samoylov, Alexandre, Anna Cochran, Bettina Schemera, Michelle Kutzler,
Caitlin Donovan, Valery Petrenko, Frank Bartol, and Tatiana Samoylova.
"Humoral immune responses against gonadotropin releasing hormone elicited
by immunization with phage-peptide constructs obtained via phage display."
Journal of Biotechnology (2015). http://www.sciencedirect.com/science/article/pii/S0168165615301498
“Abstract: Phage
display is based on genetic engineering of phage coat proteins resulting in
fusion peptides displayed on the surface of phage particles. The technology is
widely used for generation of phages with novel characteristics for numerous
applications in biomedicine and far beyond. The focus of this study was on
development of phage-peptide constructs that stimulate production of antibodies
against gonadotropin releasing hormone (GnRH). Phage-peptide constructs that
elicit production of neutralizing GnRH antibodies can be used for
anti-fertility and anti-cancer applications. Phage-GnRH constructs were
generated via selection from a phage display library using several types of
GnRH antibodies as selection targets. Such phage constructs were characterized
for sequence similarities to GnRH peptide and frequency of their occurrence in
the selection rounds. Five of the constructs with suitable characteristics were
tested in mice as a single dose 5 × 1011 virions (vir) vaccine and were found
to be able to stimulate production of GnRH-specific antibodies, but not to
suppress testosterone (indirect indicator of GnRH antibody neutralizing
properties). Next, one of the constructs was tested at a higher dose of 2 ×
1012 vir per mouse in combination with a poly(lactide- co -glycolide)
(PLGA)-based adjuvant. This resulted in multifold increase in GnRH antibody
production and significant reduction of serum testosterone, indicating that
antibodies produced in response to the phage-GnRH immunization possess
neutralizing properties. To achieve optimal immune responses for desired
applications, phage-GnRH constructs can be modified with respect to flanking
sequences of GnRH-like peptides displayed on phage. Anticipated therapeutic
effects also might be attained using optimized phage doses, a combination of
several constructs in a single treatment, or application of adjuvants and
advanced phage delivery systems. Keywords: filamentous phage; phage display;
gonadotropin releasing hormone; GnRH antibodies; contraception; hormone-dependent
reproductive cancers Abbreviations: cfu, colony forming unit; FSHR,
follicle-stimulating hormone receptor; GnRH, gonadotropin releasing hormone;
GnRHR, gonadotropin releasing hormone receptor; PLGA, poly(lactide- co
-glycolide); PEG, polyethylene glycol; vir, virion”
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