Protein phosphorylation assay kits by Tymora Analytical now available through PolySciTech

The selective phosphorylation of proteins is a key step in many pathways regulating their functions. Abnormal phosphorylation is involved in a wide variety of diseases including cancer. To perform antibody labeling, an effective antibody has to be made for each phosphorylated protein, which is an expensive and time-consuming process. Recently, Tymora Analytical has developed a titanium-based reagent assay kit to allow for detection of protein phosphorylation in a rapid, efficient, and sensitive assay. Due to a recent distribution agreement, these products are now available through PolySciTech division of Akina, Inc. (https://akinainc.com/polyscitech/products/tymora/). Learn more about this powerful assay method in a recent publication here: Iliuk, Anton, Li Li, Michael Melesse, Mark C. Hall, and W. Andy Tao. "Multiplexed Imaging of Protein Phosphorylation on Membranes Based on TiIV Functionalized Nanopolymers." ChemBioChem 17, no. 10 (2016): 900-903. http://europepmc.org/articles/4870103

“Abstract: Accurate protein phosphorylation analysis reveals dynamic cellular signaling events not evident from protein expression levels. The most dominant biochemical assay, western blotting, suffers from the inadequate availability and poor quality of phospho-specific antibodies for phosphorylated proteins. Furthermore, multiplexed assays based on antibodies are limited by steric interference between the antibodies. Here we introduce a multifunctionalized nanopolymer for the universal detection of phosphoproteins that, in combination with regular antibodies, allows multiplexed imaging and accurate determination of protein phosphorylation on membranes. Keywords: antibodies, dendrimers, membranes, multiplexed analysis, phosphoproteins”