In-vitro cytotoxicity tests are conducted as a surrogate for in-vivo testing to determine if a material has toxicity by monitoring its interactions with cells. Researchers at University of Nottingham utilized PLGA-PEG-PLGA (Cat# AK097) from PolySciTech Division of Akina, Inc. (www.polyscitech.com) to evaluate the interaction of this product with cells following the cytotoxicity tests. They found that the methodology applied for the test is important as the polymer exhibits acidic formation and other properties which yield false positives for cytotoxicity when no toxic effects are observed in animal model. This research holds promise to improve the methodology applied for evaluating toxicity of hydrogel materials. Read more: Stewart, Chloe L., Andrew L. Hook, Mischa Zelzer, Maria Marlow, and Anna M. Piccinini. "PLGA‐PEG‐PLGA hydrogels induce cytotoxicity in conventional in vitro assays." Cell Biochemistry and Function 42, no. 5 (2024): e4097. https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cbf.4097
“We identified that PLGA-PEG-PLGA hydrogels, which have been used in human clinical trials and possess a demonstrable safety profile, induced significant cytotoxicity in conventional in vitro assays. This major contradiction may lead to inconsistent and misleading toxicology due to the limited biological representation of these assays. Cytotoxicity evaluation is a crucial element of screening the biological response to new biomaterials. However, as standard test methods do not recapitulate the in vivo environment, tailored adaptations may be required to reflect the true biological response elicited toward novel biomaterials.”
PLGA-PEG-PLGA (Cat# AK097): https://akinainc.com/polyscitech/products/polyvivo/index.php?highlight=AK097#h
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