PolySciTech (www.polyscitech.com) now provides
cationic PLA and related block copolymers. These polymers have been utilized
previously for delivery of DNA/RNA as therapy for a variety of ailments. Read
more about prior utilization here: Jones, Charles H., Chih-Kuang Chen, Ming
Jiang, Lei Fang, Chong Cheng, and Blaine A. Pfeifer. "Synthesis of
cationic polylactides with tunable charge densities as nanocarriers for
effective gene delivery." Molecular pharmaceutics 10, no. 3 (2013):
1138-1145. http://pubs.acs.org/doi/full/10.1021/mp300666s
“Abstract: Well-defined cationic polylactides
(CPLAs) with tertiary amine groups were synthesized by thiol–ene click
functionalization of an allyl-functionalized polylactide to yield polymers with
tunable charge densities. CPLAs have not previously been utilized in the context
of DNA delivery. Thus, plasmid DNA (pDNA) encoding luciferase was delivered to
two physiologically distinct cell lines (macrophage and fibroblast) via
formation of CPLA/pDNA polyplexes by electrostatic interaction. The formulated
polyplexes demonstrated high levels of transfection with low levels of
cytotoxicity when compared to a positive control. Biophysical characterization
of charge densities at various CPLA/pDNA weight ratios revealed a positive
correlation between surface charge and gene delivery. Overall, these results
help to elucidate the influence of polyplex charge and size upon the delivery
of nucleic acid and support future gene delivery applications using this
next-generation biomaterial. Keywords: CPLA; polylactic acid; gene therapy;
gene delivery; DNA”
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