PolySciTech (www.polyscitech.com) provides a wide
variety of PLGA and related copolymers (PolyVivo) as well as chitosans (Kitopure). Recently these types of polymers were
utilized to design a system that allows for oral delivery of a vaccine against dysentery.
Read more: Jiang, Tao, Bijay Singh, Hui-Shan Li, You-Kyoung Kim, Sang-Kee Kang,
Jae-Woon Nah, Yun-Jaie Choi, and Chong-Su Cho. "Targeted oral delivery of
BmpB vaccine using porous PLGA microparticles coated with M cell homing
peptide-coupled chitosan." Biomaterials 35, no. 7 (2014): 2365-2373. http://www.sciencedirect.com/science/article/pii/S0142961213014397
“Abstract: M cells, the key
players of the mucosal immunity induction, are one of the intestinal barriers
for the efficient delivery of vaccines to mucosal immune tissues. To overcome
the barrier, we have developed an efficient oral vaccine carrier that
constitutes poly (lactic-co-glycolic acid) (PLGA) microparticle coated with M
cell targeting peptide. In this study, a membrane protein B of Brachyspira
hyodysenteriae (BmpB) as a model vaccine against swine dysentery was loaded
into porous PLGA microparticles (MPs). The PLGA MPs were further coated with
the water-soluble chitosan (WSC) conjugated with M cell homing peptide (CKS9)
to prepare BmpB-CKS9-WSC-PLGA MPs. Oral immunization of BmpB vaccine with
CKS9-WSC-PLGA MPs in mice showed elevated secretory IgA responses in the
mucosal tissues and systemic IgG antibody responses, providing a complete
immune response. Specifically, the immunization with these MPs demonstrated to
induce both Th1- and Th2-type responses based on elevated IgG1 and IgG2a
titers. The elevated immune responses were attributed to the enhanced M cell
targeting and transcytosis ability of CKS9-WSC-PLGA MPs to Peyer's patch
regions. The high binding affinity of CKS9-WSC-PLGA MPs with the M cells to
enter into the Peyer's patch regions of mouse small intestine was investigated
by closed ileal loop assay and it was further confirmed by confocal laser
scanning microscopy. These results suggest that the M cell targeting approach
used in this study is a promising tool for targeted oral vaccine delivery. Keywords:
M cell homing peptide; PLGA microparticles; BmpB; Oral vaccine delivery;
Chitosan”
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