PolySciTech (www.polyscitech.com)
provides a wide array of PLGA polymers. Recently these types of polymers were
coformulated along with silk, hyaluronic acid, ascorbic acid, and tetracycline
to create nanofibrous scaffold which induced stem cells to differentiate into
bone cells. This shows promise for being
able to repair severely damaged or missing bone with bone scaffolding. Read
more: Gandhimathi, Chinnasamy, Jayarama Venugopal, Seeram Ramakrishna, Samuel
Tay, and Srinivasan Kumar. "Biomimetic porous tetracycline loaded
PLGA/Silk Fibroin/Ascorbic acid/n-HA hybrid scaffolds for adipose derived stem
cells differentiation into Osteogenic lineage (LB32)." The FASEB Journal
28, no. 1 Supplement (2014): LB32. http://www.fasebj.org/content/28/1_Supplement/LB32.short
“Abstract: The objective of this study is to fabricate poly
(D,L-lactide-co-glycolide) (PLGA)/Silk fibroin(SF)/Ascorbic
acid(AA)/Tetracycline (TC) nanofibrous scaffolds and nanohydroxyapatite (n-HA)
was deposited by calcium phosphate dipping method. These nanofibrous scaffolds
were characterized using scanning electron microscopy (SEM), surface
wettability, functional groups analysis, porosity and tensile properties.
Adipose derived stem cells (ADSCs) were cultured on these nanofibrous scaffolds
and were made to undergo osteogenic differentiation in the presence of TC/n-HA.
Osteogenic differentiation of ADSCs was confirmed using alkaline phosphatase
activity (ALP), mineralization (ARS) and immunofluorescent staining using both
ADSC specific marker protein CD105 and osteoblast specific marker protein
osteocalcin. SEM micrographs of PLGA/SF/AA/TC/n-HA fabricated electrospun fiber
diameters measured around 430 ± 26 nm to 220 ± 27 nm and FT-IR analysis showed
the amide I, II and III groups. The obtained PLGA/SF/AA/TC/n-HA scaffolds were
hydrophilic, having water contact angle of 0° compared to PLGA nanofibers
(119.2 ± 12.11°). Resulting nanofibrous scaffolds were highly porous (85-91%)
and provided desirable architecture for transport of nutrients and metabolic
waste and facilitate neovascularization. The PLGA/SF/AA/TC/n-HA scaffolds had
mechanical properties comparable to that of native bone with tensile break of
28.52% and Young’s modulus of 2.94 MPa, while that of PLGA nanofibers was
comparatively lower at 1.96 ± 0.68 MPa. Cell culture studies performed on the
bioactive AA/TC/n-HA molecules were incorporated on the nanofibers to develop
specific biological functions like proliferation, mineralization and
differentiation of ADSCs into osteogenic lineage. The results proved that the
ADSCs differentiated cells showed osteoblast-like morphology, expression of
osteocalcin and mineralization. Overall the data suggest that the abundance and
accessibility of biodegradable PLGA/SF/AA/TC/n-HA nanofibrous scaffolds can be
used as a carrier for the sustained release of biomolecules and promote greater
osteogenic differentiation of ADSCs, proving them to be potential scaffolds for
bone tissue engineering.”
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