PolySciTech (
www.polyscitech.com)
provides a wide array of block copolymers including reactive endcapped
maleimide-PEG-PLA and PLGA type copolymers. The maleimide functionality allows
for conjugation to thiol in pH neutral aqeous solution allowing for
peptide/protein conjugation. Recently this was utilized to generate a
nanoparticle which has a peptide targeting moiety towards brain cancer cells.
It was found that this nanoparticle successfully delivered chemotherapeutic
paclitaxel to these cells in mouse test. Read more: Zhang, Bo, Shun Shen, Ziwei
Liao, Wei Shi, Yu Wang, Jingjing Zhao, Yue Hu et al. "Targeting
fibronectins of glioma extracellular matrix by CLT1 peptide-conjugated
nanoparticles." Biomaterials 35, no. 13 (2014): 4088-4098. http://www.sciencedirect.com/science/article/pii/S0142961214000623
“Abstract: The abundant extracellular matrix
(ECM) in the glioma microenvironment play a critical role in the maintenance of
glioma morphology, glioma cells differentiation and proliferation, but little
has been done to understand the feasibility of ECM as the therapeutic target
for glioma therapy. In this study, a drug delivery system targeting
fibronectins (FNs), a prevailing component in the ECM of many solid tumors, was
constructed for glioma therapy based on the interaction between the abundant
FNs in glioma tissues and the FNs-targeting moiety CLT1 peptide. CLT1 peptide
was successfully conjugated to PEG-PLA nanoparticles (CNP). FNs were
demonstrated to be highly expressed in the ECM of glioma spheroids in vitro and
glioma tissues in vivo. CLT1 modification favored targeting nanoparticles
penetration into the core of glioma spheroids and consequently induced more
severe inhibitive effects on glioma spheroids growth than traditional NP. In
vivo imaging, ex vivo imaging and glioma tissue slides showed that CNP enhanced
nanoparticles retention in glioma site, distributed more extensively and more
deeply into glioma tissues than that of conventional NP, and mainly located in
glioma cells rather than in extracellular matrix as conventional NP.
Pharmacodynamics outcomes revealed that the median survival time of
glioma-bearing mice models treated with paclitaxel-loaded CNP (CNP-PTX) was
significantly prolonged when compared with that of any other group. TUNEL assay
demonstrated that more extensive cell apoptosis was induced by CNP-PTX
treatment compared with other treatments. Altogether, these promising results
indicated that this ECM-targeting drug delivery system enhanced retention and
glioma cell uptake of nanoparticles and might have a great potential for glioma
therapy in clinical applications. Keywords: Extracellular matrix; Fibronectin;
Targeting; Nanoparticles; Glioma; Retention”
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