PolySciTech (
www.polyscitech.com)
provides a wide variety of PLGA-PEG-PLGA triblock thermogels for use as drug
delivery depots. Recently researchers have utilized similar polymers to deliver
SiRNA and doxorubicin to osterosarcoma cells successfully inducing apoptosis
both in-vitro and in nude mice. Read more: Ma, Hecheng, Chaoliang He, Yilong
Cheng, Dongsong Li, Yubao Gong, Jianguo Liu, Huayu Tian, and Xuesi Chen.
"PLK1shRNA and doxorubicin co-loaded thermosensitive PLGA-PEG-PLGA
hydrogels for osteosarcoma treatment." Biomaterials 35, no. 30 (2014):
8723-8734.
http://www.sciencedirect.com/science/article/pii/S0142961214007510
“Abstract: Combination cancer therapy has
emerged as crucial approach for achieving superior anti-cancer efficacy. In
this study, we developed a strategy by localized co-delivery of
PLK1shRNA/polylysine-modified polyethylenimine (PEI-Lys) complexes and
doxorubicin (DOX) using biodegradable, thermosensitive PLGA-PEG-PLGA hydrogels
for treatment of osteosarcoma. When incubated with osteosarcoma Saos-2 and
MG-63 cells, the hydrogel containing PLK1shRNA/PEI-Lys and DOX displayed
significant synergistic effects in promoting the apoptosis of osteosarcoma
cells in vitro. After subcutaneous injection of the hydrogel containing
PLK1shRNA/PEI-Lys and DOX beside the tumors of nude mice bearing osteosarcoma
Saos-2 xenografts, the hydrogels exhibited superior antitumor efficacy in vivo
compared to the hydrogels loaded with PLK1shRNA/PEI-Lys or DOX alone. It is
noteworthy that the combination treatment in vivo led to almost complete
suppression of tumor growth up to 16 days, significantly enhanced PLK1
silencing, higher apoptosis of tumor masses, as well as increased cell cycle
regulation. Additionally, ex vivo histological analysis of major organs of the
mice indicated that the localized treatments showed no obvious damage to the
organs, suggesting lower systemic toxicity of the treatments. Therefore, the
strategy of localized, sustained co-delivery of PLK1shRNA and DOX by using the
biodegradable, injectable hydrogel may have potential for efficient clinical
treatment of osteosarcoma. Keywords: Combination therapy; PLK1 silencing; Synergistic
effect; Localized delivery; Injectable hydrogel; Osteosarcoma”
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