PolySciTech (
www.polyscitech.com)
provides a wide array of PEG-PLA as well as other polymers. Recently gene
loaded nanoparticles were utilized as part of a delivery system to control the
delivery of DNA to cervical cancer cells. Read more Liu, Bo, Shu-Mei Han,
Xiao-Yong Tang, Li Han, and Chang-Zhong Li. "Cervical Cancer Gene Therapy
by Gene Loaded PEG-PLA Nanomedicine." Asian Pacific Journal of Cancer
Prevention 15, no. 12 (2014): 4915-4918. Full-Text available: http://www.apocpcontrol.org/paper_file/issue_abs/Volume15_No12/4915-4918%204.6%20Bo%20Liu.pdf
“Abstract: Background and Aims: Advances in the
treatment of cervical cancer over the last decade have predominantly involved
the development of genes directed at molecular targets. Gene therapy is
recognized to be a novel method for the treatment of cervical cancer. Genes can
be administered into target cells via nanocarriers. This study aimed to develop
systemically administrable nano-vectors. Folate (Fa) containing gene loaded
nanoparticles (NPs) could target HeLa human cervical cancer cells through
combination with receptors on the cells to increase the nuclear uptake of
genetic materials. Methods: Fa was linked onto Poly (ethylene glycol)-b-poly
(D, L-lactide) (PEG-PLA) to form Fa-PEG-PLA, and the resulting material was
used to load plasmids of enhanced green fluorescence protein (pEGFP) to obtain
gene loaded nanoparticles (Fa-NPs/DNA). Physical-chemical characteristics, in vitro
release and cytotoxicity of Fa-NPs/DNA were evaluated. The in vitro
transfection efficiency of Fa-NPs/ DNA was evaluated in HeLa cells and human
umbilical vein endothelial cells (HUVEC). PEG-PLA without Fa was used to load
pEGFP from NPs/DNA as a control. Results: Fa-NPs/DNA has a particle size of 183
nm and a gene loading quantity of 92%. After 72h of transfection, Fa-NPs/DNA
displayed over 20% higher transfection efficiency than NPs/DNA and 40% higher
than naked DNA in HeLa cells. However, in HUVECs, no significant difference
appeared between Fa-NPs/DNA and NPs/DNA. Conclusions: Fa-PEG-PLA NPs could function
as excellent materials for gene loading. This nano-approach could be used as
tumor cell targeted medicine for the treatment of cervical cancer. Keywords:
Gene therapy - cervical cancer - folate coating - targeted nanomedicine”
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